A technique for separating nucleic acids or proteins on the basis of their size and electrical charge, both of which affect their rate of movement through an electric field in a gel made of agarose or another polymer.
A small, circular, double-stranded dna molecule that carries accessory genes separate from those of a bacterial chromosome; in dna cloning, plasmids are used as vectors carrying up to about 10,000 base pairs (10 kb) of DNA. Plasmids are also found in some eukaryotes, such as yeasts.
In genetic engineering, a DNA molecule that can carry foreign DNA into a host cell and replicate there. Cloning vectors include plasmids and bacterial artificial chromosomes (BACs), which move recombinant DNA from a test tube back into a cell, and viruses that transfer recombinant DNA by infection.
Technology for sequencing and manipulating DNA.
Embryonic stem cells capable of differentiating into many different cell types.
A DNA segment that results from the cutting of DNA by a restriction enzyme.
The production of multiple copies of a specific DNA segment.
A single-stranded end of double-stranded restriction fragment.
Down
An endonuclease (type of enzyme) that recognizes and cuts DNA molecules foreign to a bacterium (such as phage genomes). The enzyme cuts at specific nucleotide sequences (restriction sites).
Determine the complete nucleotide sequence of a gene or DNA segment.
A specific sequence on a DNA strand that is recognized and cut by a restriction enzyme.
The manipulation of organisms or their components to produce useful products.
Any relatively unspecialized cell that can produce, during a single division, two identical cells.
The production of multiple copies of a gene.
A cell that can generate a complete new organism.
A technique for amplifying DNA in vitro by incubating it with specific primers, a heat-resistant DNA polymerase, and nucleotides.
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Quick Answers:
Where is my puzzle?
All your puzzles are accessible from your 'My Puzzles' page, which you can access using the navigation bar at the top when you are logged in.
Be sure to log in using the same email address you used when you created your puzzle.
How do I print?
There is a 'Make Printable' button on the top left of your puzzle that will let you sign up for a plan or purchase a single puzzle.
Once paid, that button will turn into a 'Preview + Publish' button that will put your puzzle in a format that can be printed or solved online.
Once you publish your puzzle, you can click the print icon or use your browser’s print function.
It looks weird when printed.
First make sure you’ve published your puzzle. See the 'How do I print?' section above for more information.
99% of other printing issues have to do with printer settings. Instead of trying to fiddle with printer settings, which can be time-consuming and frustrating, there are a couple workarounds you can try.
You can try printing from a different browser, since different browsers have different default print settings. Alternately, you can try saving as a PDF (next to the print icon) and print that.
Why isn’t this free?
We invested in building a number of premium features that free sites are unable to offer: the ability to automatically or manually add words to your puzzle, save-as-you-go puzzle editing, the ability to access your puzzles from any computer, an uncluttered and ad-free interface, the ability for friends and colleagues to solve your puzzles online, and quick responsiveness to reported issues.
My Crossword Maker is the lowest-price service we've found that provides these features, and is designed for people who are not satisfied with what free sites are able to provide.
I thought this was free.
We’re sorry you thought My Crossword Maker was free, that must have been frustrating.
We’re constantly trying to provide a clear and straightforward user experience, and we’re not trying to hide the price: on the page where you make your puzzle, the price is listed in the overview in the top left (before you log in), below the 'Make Printable' button (after you log in), in the FAQ below the grid, and also in the tour.
Unfortunately, we have limited control over how we show up in Google and on other sites, so if they show My Crossword Maker when someone searches for a free site we can’t do anything about that.
It says there is no account with my email.
First check whether you used a different email address to create your account. Your login email is the email address where you received a welcome email from us.
Alternately, it’s possible there was a typo in your email when you created your account. If so, report an issue below and provide the name and last four digits on the card you used when you signed up. We’ll use this information to track down your account.
I am being asked to pay a second time.
If you are a member of My Crossword Maker and are still being asked for payment, then you may have inadvertently created a second account.
Your paid account will have the email address where you received an email confirming your subscription.
If you never received such an email, or are still unable to find your paid account, report an issue below and provide the name and last four digits on the card you used when you signed up. We’ll use this information to track down your account.
My password reset isn’t working.
For security a human can’t see or reset your password, so read the below information carefully.
If you haven’t gotten the password reset email, check your spam folder, be sure you’re checking the email account that the email was sent to, and be aware that some school email systems block emails from non-school email addresses.
If you have the password reset email but the password isn’t accepted, be sure you are using the most recent password reset email: sometimes people will hit the button twice, and only the second password will work.
Be sure if you copy-paste the password not to get a space on the outside, or if you're typing it keep in mind that it's case-sensitive and that a lower-case L can look like the number 1, etc.
How do I cancel?
You can cancel your subscription from your 'My Account' page, which you can access using the navigation bar at the top when you are logged in.
Support representatives cannot cancel a subscription on your behalf.
Not all my words are in my puzzle.
When you hit the 'Arrange' button, our algorithm will try to arrange all your words on the grid, and will move words around to try to fit the most words.
This will get all the words on the grid about 90% of the time, depending on the word list. For the other 10%, you will get an alert explaining that the words that did not fit were added to the scratchpad.
It prints on two pages.
Big puzzles won’t always print on one page — the clues and grid are the smallest we could make them while keeping them readable for everyone.
That said, different browsers print in slightly different ways, so you could try using 'print preview' using a different browser (or try the PDF button) to see if you like how that looks better.
I didn’t hear back from you.
We generally respond to all reported issues in about a day. If it’s been a few days and you haven’t heard back from us, it’s possible we sent an email that didn’t reach you.
Check your spam folder, and be aware that some school email systems block emails from non-school email addresses. Try providing a different email address if you think our emails to you are being blocked.
Answers take about a day. Try the Quick Answers above for a faster resolution.
Where is my puzzle?