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GacS/GacA Two-component regulatory system and RNAs as regulators

Across
Example of a target gene repressed by RsmA in GacS/GacA 2-component system
Biofilm formation is a result of which type of infection
Secretion system containing needle complexes that allows bacterial proteins to be secreted directly into host cytoplasm
Maximum secondary metabolite production occurs during which cell cycle phase
Gacs forms a heterodimer with ___ during acute infection states
RsmA blocks ___ of target genes
Gac-box is located - ____ bp from TS start (roman numerals)
Phosphorylated ___ induces TS of rsmX, rsmY, rsmZ
P.a. uses ___ SKs as genetic switch for reciprocal regulation
c-di-GMP ___ controls T3SS & T6SS
RsmA activates ___ genes - essential for biofilms
Gram - soil isolate
downregulation of aprA-lacZ causes cells to appear
rsmX, rsmY and rsmZ are small ___ RNAs
71% a.a. sequence similarity to RsmA
RetS mutant over produces exopolysaccharides and ___
RsmA ribosome-binding site
Rsm system controls genes for antibiotics and ___
Chronic infections lead to a ___ in virulence
Example of a diguanylate cyclase that makes c-di-GMP
RetS ___ formation of GacS homodimers
Secretion system active in P. aeruginosa chronic infection of cystic fibrosis (CF) patients
Down
Rsm controls genes at which level of regulation
Second messenger used in p.a. to transition between sessile and planktonic lifestyle
Secondary metabolites may protect against ___ diseases
Phosphorylated GacA binds DNA via its ___ motif
Type of interaction occurring between GacS and RetS
Phosphorylated GacgA is required for TS of ___
Functions by titrating out RsmA/E repressor proteins (1 example)
Growth phase in which secondary metabolites are produced
Antagonizes RsmA
Gene responsible for hydrogen cyanide synthesis
GacS/A is a 2-component system that is useful for ___
One reason RNAs are used for regulation over proteins
1 biosynthetic operon for polysaccharide synthesis
Low c-di-GMP results in ___ & virulence
AprA is responsible for the synthesis of ___ protease
During chronic infection, GacS is in its ___ configuration
RsmA positively regulates T3SS, likely by ___ control
RetS and ___ act antagonistically to control transition between 2 infection types
RsmA represses ___ genes (1 example)
Titrated out by SRNAs
T3SS increases motility during ___ infections