Biofilm is a ___ community of cells attached to a substratum embedded in an EPS matrix
Facilitates colonization
Gram - bacteria form antibiotic resistant biofilms in response to
Downregulation of fliC during biofilm formation causes cells to lose their ___
DsrA acts in ___ to regulate 2 different mRNAs
Tobramycin increases swimming/swarming motility of P. aeruginosa at ___ antibiotic concentrations
Determinants of specificity for antisense/RNA target pairing
Complete inhibition of replication occurs when [ColE1] reaches ___ copies per cell (roman numerals)
When RNA gene overlaps with target gene, it is said to be (2 words)
Gram + soil bacteria
Forms RNA-DNA hybrid at replication origin
Cells in biofilms are 1000x more resistant to antibiotics than ___ cells
Antibiotics function as mediators of intercellular signaling when concentrations are ___
Antisense RNA that negatively regulates ompF
Example of a beneficial biofilm (2 words) ___ ___ treatment
Needle-like projections to detect eukaryotic organisms and directly inject bacterial proteins
Stress response sigma
Small, diffusable, untranslated RNA (2words)
Down
TS activator os T3SS
Example of a + charged antibiotic which sticks to - charged EPS
Repressed by DsrA
Gene that is upregulated during biofilm formation
Example of a detrimental biofilm affecting the ear (2 words)
RNA stems determine (2 words)
Type III secretion system plays a role in the ___ phenotype of P. aeruginosa
RNAI is synthesized from ColE1 ___
Second step of biofilm formation is the formation of ___
Protein that forms a dimer and enhances pairing between RNAI and RNAII
Average number of a particular plasmid per cell is called the (2 words) ___ ___ #
MicF is located ___ of ompC
Regulatory RNA alters expression/function of RNA post-____
When antisense and target RNA are expressed from unlinked genes, complementarity is said to be
In order to act as a primer for replication, RNAII must be cleaved by
T3SS secreted exotoxin
Plasmid copy number determined by
87-nt regulatory RNA
Inhibits plasmid replication by interfering with processing of RNAII
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