Traditional procedure which detects the presence of a specific antigen-antibody reaction by causing the in in vitro activation of complement. If complement is not fixed, lysis of preantibody-coated reagent RBCs occurs.
General term for the quantitative testing of both antigens and antibodies which uses enzyme substrate to measure Ag-Ab reactions
A nonspecific, acute-phase reactant glycoprotein
Laboratory technique for the detection of antibodies that involves the agglutination of RBCs.
- A procedure in which oppositely charged antigen and antibody are propelled toward each other by an electrical field
A specimen such as serum with known assay values that is tested concurrently with the unknown patient specimens
Procedure using the visual detection of fluorescent dyes conjugated to antibodies that react with antigen.
Method for the quantitative study of antibodies (RID) or qualitative identity of antigens (Ouchterlony technique).
Promotes agglutination of RBCs that are sensitized with IGG or complement.
Method that uses RBCs passively coated with antigens to detect antibodies
Most abundant coagulation factor the forms fibrin clots to help serve as barrier to prevent spread of infection.
Antigen excess
Negative charged electrode that attracts positive charged particles.
Down
Double angular immonodiffusion
Positive charged electrode that attracts negative charged particles
Light emitted as a consequence of a chemical reaction.
Two step procedure involving the electrical separation of proteins, followed by the linear diffusion (immunofixation)of antibodies into the electrophoresis gel from a trough that extends through the length of the gel adjacent to the electrophoretic path. Precipitin arcs are produced at the equivalence point.
A dye-antibody combination that emits light of another longer wavelength.
Technique for detecting antibodies that involves the blocking of agglutination of RBCs
Strength with which a multivalent antibody binds to a multivalent antigen.
The relative concentration of antibody and antigen that produces the maximal binding of antibody to antigen.
The concentration or strength of an antibody expressed in the highest dilution of the serum that produces agglutination.
Antibody excess
Enzyme-linked immunosorbent assay
Immunoflourescent assay
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