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MIC 446

Across
A gel is transferred to what for a Western?
blue/white screening depends on the chemical reaction catalyzed by this enzyme
Chemical used after coomassie dye.
Light source used to visualize gels.
Device used to heat tubes.
What liquid do you soak your Western in that contains milk?
the total volume of a molecular-biology sized 1:4 dilution
a spectrophotometric peak at 280 nm is indicative of
Device used to spin things down.
without this component of solution I, a gel of your miniprep will have many small contaminant bands
typical serum albumin control comes from this animal
Chemical used to clean lab bench.
What is the packing technique called to run a Western?
three stop codons in alphabetical order
Run with your samples on a gel to determine band size
Down
Make specific cuts in a sequence.
buffer essential to this technique contains CAPS and methanol
essential component of solution III to allow for renaturation of plasmid DNA
The process used to glue sequences together.
Where you keep all your protocols.
Device used to mix things rapidly.
the part of an SDS-PAGE gel that requires a higher concentration of acrylamide
Method using primers to multiply sequence of interest.
Diagnostic to determine if Lysates worked.
Protective clothing worn in the lab