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Flow Cytometry Basics

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Across
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Location of interrogation point for event in a flowing stream
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Flow Cytometry Subsystem that includes excitation and emission components
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This data display uses a two dimensional plotting strategy, plotting a dot for every event
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Acronym for parameter that can be used to determine relative complexity or granularity of a particle
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Optimal pulse measurement
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Flow Cytometry samples must be in this form
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Acronym for light measured "forward" of the LASER
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This type of plot has # of events on the y-axis
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This is monochromatic and unidirectional
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T cells and B cells are these
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Measurement in Flow Cytometry that can be inherent to a particle or due to an attached tag
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Done after data acquisition
Down
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Flow rate that should be used for low CVs
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This pulse measurement is used when applying a threshold
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Flow Cytometry Subsystem that converts photons into data
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Flow Cytometry Subsystem that delivers cells to interrogation point
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A population that can be identified by FSC and SSC alone
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When a particle goes through a LASER, this electronic result is created. It is represented by a plot of Time vs. Volts.
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A filter that stipulates the wavelengths that pass through by using the following format "488/10"
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Optical filter that allows long wavelengths to pass
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This biological sample cannot be acquired on a flow cytometer without proper preparation
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Quadrant where double positive events fall in a two-dimensional plot
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Name for a test that measures 3 major lymphocyte populations
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A technology that simultaneously measures multiple characteristics of single particles, serially, in a rapidly flowing stream
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Force required for fluid stream on a FACSCanto